CURRENT PROTOCOLS IN BIOINFORMATICS
CURRENT PROTOCOLS IN CELL BIOLOGY
CURRENT PROTOCOLS IN CYTOMETRY
CURRENT PROTOCOLS IN FIELD ANALYTICAL CHEMISTRY
CURRENT PROTOCOLS IN FOOD ANALYTICAL CHEMISTRY
CURRENT PROTOCOLS IN HUMAN GENETICS
CURRENT PROTOCOLS IN IMMUNOLOGY
CURRENT PROTOCOLS IN MAGNETIC RESONANCE IMAGING
CURRENT PROTOCOLS IN MOLECULAR BIOLOGY
    FRONT MATTER
    CHAPTER 1. ESCHERICHIA COLI, PLASMIDS, AND BACTERIOPHAGES
    CHAPTER 2. PREPARATION AND ANALYSIS OF DNA
    CHAPTER 3. ENZYMATIC MANIPULATION OF DNA AND RNA
    CHAPTER 4. PREPARATION AND ANALYSIS OF RNA
    CHAPTER 5. CONSTRUCTION OF RECOMBINANT DNA LIBRARIES
    CHAPTER 6. SCREENING OF RECOMBINANT DNA LIBRARIES
    CHAPTER 7. DNA SEQUENCING
    CHAPTER 8. MUTAGENESIS OF CLONED DNA
    CHAPTER 9. INTRODUCTION OF DNA INTO MAMMALIAN CELLS
    CHAPTER 10. ANALYSIS OF PROTEINS
    CHAPTER 11. IMMUNOLOGY
    CHAPTER 12. DNA-PROTEIN INTERACTIONS
    CHAPTER 13. YEAST
    CHAPTER 14. IN SITU HYBRIDIZATION AND IMMUNOHISTOCHEMISTRY
    CHAPTER 15. THE POLYMERASE CHAIN REACTION
    CHAPTER 16. PROTEIN EXPRESSION
    CHAPTER 17. PREPARATION AND ANALYSIS OF GLYCOCONJUGATES
    CHAPTER 18. ANALYSIS OF PROTEIN PHOSPHORYLATION
    CHAPTER 19. INFORMATICS FOR MOLECULAR BIOLOGISTS
    CHAPTER 20. ANALYSIS OF PROTEIN INTERACTIONS
            CHAPTER 20. INTRODUCTION
            UNIT 20.1 Interaction Trap/Two-Hybrid System to Identify Interacting Proteins
            UNIT 20.2 Affinity Purification of Proteins Binding to GST Fusion Proteins
            UNIT 20.3 Phage-Based Expression Cloning to Identify Interacting Proteins
            UNIT 20.4 Surface Plasmon Resonance for Measurements of Biological Interest
            UNIT 20.5 Detection of Protein-Protein Interactions by Coprecipitation
            UNIT 20.6 Identification of Protein Interactions by Far Western Analysis
            UNIT 20.7 Two-Hybrid Dual Bait System
                INTRODUCTION
                BASIC PROTOCOL 1: PREPARATION OF BAITS AND LIBRARY: CHARACTERIZING BAIT PROTEINS
                BASIC PROTOCOL 2: PREPARATION OF BAITS AND LIBRARY: TRANSFORMING AND CHARACTERIZING THE LIBRARY
                BASIC PROTOCOL 3: SELECTING AN INTERACTOR
                BASIC PROTOCOL 4: ANALYSIS OF PRIMARY INTERACTORS
                ALTERNATE PROTOCOL: SCREENING FOR INTERACTION TRAP POSITIVES BY YEAST PLASMID RECOVERY
                SUPPORT PROTOCOL 1: COLORIMETRIC ASSAY OF b-GALACTOSIDASE AND b-GLUCURONIDASE ACTIVITY BY AGAROSE OVERLAY
                SUPPORT PROTOCOL 2: DETECTION OF BAIT PROTEIN EXPRESSION
                SUPPORT PROTOCOL 3: PREPARATION OF HIGH-QUALITY DIGESTED LIBRARY PLASMID
                COMMENTARY
                Literature Cited
                Contributors
                FIGURE(S)
                    Figure 20.7.1 Outline of the dual bait system and the key control points.
                    Figure 20.7.2 Flow chart of the two-hybrid screen done by interaction mating.
                    Figure 20.7.3 Plasmid map of the 10101-nt basic dual bait vector pMW103.
                    Figure 20.7.4 Polylinkers of the two-hybrid basic vectors.
                    Figure 20.7.5 Plasmid map of the 5310-nt basic dual bait vector pGBS10.
                    Figure 20.7.6 Plasmid map of the 11099-nt basic dual bait vector pLacGus, which is also known as pDR8.
                    Figure 20.7.7 Detailed library screening flow chart.
                    Figure 20.7.8 Plasmid map of the basic dual bait vector pJG4-5.
                    Figure 20.7.9 Detailed flow chart for characterization and second confirmation of primary positives.
                TABLE(S)
    CHAPTER 21. CHROMATIN ASSEMBLY AND ANALYSIS
    CHAPTER 22. NUCLEIC ACID ARRAYS
    CHAPTER 23. MANIPULATING THE MOUSE GENOME
    CHAPTER 24. GENERATION AND USE OF COMBINATORIAL LIBRARIES
    CHAPTER 25. DISCOVERY AND ANALYSIS OF DIFFERENTIALLY EXPRESSED GENES IN SINGLE CELLS AND CELL POPULATIONS
    CHAPTER 26. GENE SILENCING
    CHAPTER 27. RNA-PROTEIN INTERACTIONS
    APPENDIX 1 STANDARD MEASUREMENTS, DATA, AND ABBREVIATIONS
    APPENDIX 2 COMMONLY USED REAGENTS AND EQUIPMENT
    APPENDIX 3 COMMONLY USED TECHNIQUES IN BIOCHEMISTRY AND MOLECULAR BIOLOGY
    APPENDIX 4 SELECTED SUPPLIERS OF REAGENTS AND EQUIPMENT
    APPENDIX 5 VECTORS
CURRENT PROTOCOLS IN NEUROSCIENCE
CURRENT PROTOCOLS IN NUCLEIC ACID CHEMISTRY
CURRENT PROTOCOLS IN PHARMACOLOGY
CURRENT PROTOCOLS IN PROTEIN SCIENCE
CURRENT PROTOCOLS IN TOXICOLOGY