CHAPTER 16. PROTEIN EXPRESSION
SECTION I EXPRESSION OF PROTEINS IN ESCHERICHIA COLI
UNIT 16.2 Expression Using the T7 RNA Polymerase/Promoter System
FIGURE(S)
Figure 16.2.1 pT7-5, pT7-6, and pT7-7.
 Figure 16.2.1 pT7-5, pT7-6, and pT7-7. pT7-5, pT7-6, and pT7-7 are cloning vectors that contain a T7 promoter and are used to express genes using T7 RNA polymerase. All three vectors contain a T7 RNA polymerase promoter, the gene encoding resistance to the antibiotic ampicillin and the ColE1 origin of replication. pT7-7 has a strong ribosome-binding site (rbs) and start codon (ATG) upstream of the polylinker sequence; the sequence of this region is shown below the map of pT7-7. pT7-5 and pT7-6 lack any ribosome-binding site upstream of the polylinker sequence and consequently are only useful when expressing genes that already contain the proper control sequences. pT7-5, pT7-6, and pT7-7 were constructed by S. Tabor and are derivatives of pT7-1 described in Tabor and Richardson (1985). From Current Protocols in Molecular Biology Online Copyright © 2003 John Wiley & Sons, Inc. All rights reserved. |