CURRENT PROTOCOLS IN MOLECULAR BIOLOGY
  CHAPTER 16. PROTEIN EXPRESSION
    SECTION I EXPRESSION OF PROTEINS IN ESCHERICHIA COLI
      UNIT 16.2 Expression Using the T7 RNA Polymerase/Promoter System
        FIGURE(S)
          Figure 16.2.1 pT7-5, pT7-6, and pT7-7.


Figure 16.2.1 pT7-5, pT7-6, and pT7-7. pT7-5, pT7-6, and pT7-7 are cloning vectors that contain a T7 promoter and are used to express genes using T7 RNA polymerase. All three vectors contain a T7 RNA polymerase promoter, the gene encoding resistance to the antibiotic ampicillin and the ColE1 origin of replication. pT7-7 has a strong ribosome-binding site (rbs) and start codon (ATG) upstream of the polylinker sequence; the sequence of this region is shown below the map of pT7-7. pT7-5 and pT7-6 lack any ribosome-binding site upstream of the polylinker sequence and consequently are only useful when expressing genes that already contain the proper control sequences. pT7-5, pT7-6, and pT7-7 were constructed by S. Tabor and are derivatives of pT7-1 described in Tabor and Richardson (1985).


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